FISP probes

Sterile In-Situ Fermentation/Bioreactor Sampling Probe

Withdraws Sterile, Cell-Free Samples From Fermentors, Bioreactors or Other Vessel Types

Flownamics® has developed the FISP®, which is a sampling probe capable of withdrawing sterile, cell-free samples from fermentors and bioreactors. FISP allows direct on-line sample transfer to a variety of analyzers, such as biochemistry and HPLC systems, as well as collection for off-line analysis. FISP® is a small, tube-shaped, sterilizable 316 stainless steel carrier which is surrounded by a tubular, micro-porous membrane. They are available for 12, 19 and 25 mm ports for use in laboratory, pilot or industrial scale vessels. FISP® has been and continues to be widely utilized in the fermentation and cell culture fields since it’s introduction to the market in 1996.


Microbial and Mammalian Sampling

FISP will perform well whether the process is a microbial fermentation requiring multiple samples per hour, or a mammalian cell culture requiring less frequent sampling. Sampling with FISP provides an excellent means of providing on-line nutrient and metabolite monitoring of bacterial, yeast, fungal, and mammalian cell cultures. Data obtained from using the FISP increases process understanding while improving process monitoring and control.

Operation of the FISP®

The FISP is inserted into a side or top port of the vessel. It is immersed into the fermentation broth or culture media and sterilized in-situ. A peristaltic pump (or the vessel’s own hydrostatic pressure) withdraws a sample through the tubular membrane and into the probe’s stainless steel network of grooves. The sample flows through the network of grooves into a central channel where the cell-free sample can be directed to an on-line analyzer, fraction collector, or sample container. Fluid flow rate is dependent on the viscosity of the media.

FISP operation
FISP in action

Features of the FISP®

  • Sterile, cell-free sampling for on-line or off-line analysis
  • Autoclave, SIP, & CIP compatible
  • Risk free sampling with 0.2 micron filter (sterile barrier)
  • Fits small laboratory to large full-scale production vessels
  • Collect and store samples for off-line analysis
  • Minimal dead volume for consistent and accurate sampling, which reduces flush time
  • Withstands the temperatures, pressures, viscosities, shear forces, and chemicals typical of fermentation environments
  • Use with aerobic or anaerobic bacteria, yeast, fungi, algae, insect, and mammalian cell cultures
  • Easy to use and maintain; no operator training required
  • Can be used for submerged feeding/sterile media additionInterfaces with our Seg-Flow® Sampling System (additional information available)
  • Directly interfaces the fermentor or bioreactor with:
    • Biochemistry analyzers, Fraction collectors, Autosamplers, Flow Injection Analysis (FIA) systems, HPLC systems, Biosensors, Methanol analyzers
FISP F-series probes

FISP® F-Series

F-Series Features

  • Available For 12 & 19mm Ports
  • Fits Into the Fermenter / Bioreactor Top Port
  • Compatible with Kleenpak™ connectors
  • Can be used with SUBs
  • Dead Volume: Approximately 0.24 to 0.44ml
  • Immersion Depths: 120, 200, 310, & 410mm
  • 0.2 μm membrane (custom pore sizes available)
FISP D-series probes

FISP® D-Series

D-Series Features

  • Available For 25mm Ports: Standard & Safety
  • Fits Into the Fermenter / Bioreactor Side Port
  • Dead Volume: Approximately 0.24 to 0.44ml
  • Used in PD to production
  • GMP Validated
  • Immersion Depths: 90 & 115mm
  • 0.2 μm membrane (custom pore sizes available)